Expanding Human Blood Stem Cells with Garcinol
Author Information
Author(s): Nishino Taito, Wang Changshan, Mochizuki-Kashio Makiko, Osawa Mitsujiro, Nakauchi Hiromitsu, Iwama Atsushi
Primary Institution: Chiba University
Hypothesis
Can Garcinol, a histone acetyltransferase inhibitor, effectively expand human hematopoietic stem cells ex vivo?
Conclusion
Garcinol significantly increases the number of hematopoietic stem cells and progenitor cells in culture.
Supporting Evidence
- Garcinol increased the number of CD34+CD38– HSCs/PCs more than 4.5-fold.
- Isogarcinol, a derivative of Garcinol, expanded HSCs/PCs 7.4-fold.
- Garcinol expanded the number of SCID-repopulating cells 2.5-fold.
Takeaway
Garcinol helps grow more blood stem cells from umbilical cord blood, which can be useful for treatments.
Methodology
The study screened 92 natural products for their ability to expand hematopoietic stem cells and found that Garcinol increased cell numbers significantly during a 7-day culture.
Participant Demographics
Human cord blood samples were used.
Statistical Information
P-Value
0.019
Confidence Interval
95% confidence interval of 1 of 14,109 to 1 of 8,453
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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