Oxymatrine and its effects on pancreatic cancer cells
Author Information
Author(s): Ling Qi, Xu Xiao, Wei Xuyong, Wang Weibing, Zhou Bin, Wang Bei, Zheng Shusen
Primary Institution: First Affiliated Hospital, Zhejiang University School of Medicine
Hypothesis
The study investigates the anti-cancer effect of oxymatrine on human pancreatic cancer PANC-1 cells and its possible molecular mechanism.
Conclusion
Oxymatrine can induce apoptotic cell death of human pancreatic cancer cells by regulating Bcl-2 and IAP families, releasing cytochrome c, and activating caspase-3.
Supporting Evidence
- Oxymatrine inhibited cell viability and induced apoptosis of PANC-1 cells in a time- and dose-dependent manner.
- The expression of Livin and Survivin genes was down-regulated while the Bax/Bcl-2 ratio was upregulated.
- Oxymatrine treatment led to the release of cytochrome c and activation of caspase-3 proteins.
Takeaway
Oxymatrine, a substance from a traditional Chinese herb, can help kill pancreatic cancer cells by making them die in a controlled way.
Methodology
The study used MTT assay, flow cytometry, RT-PCR, and Western blotting to assess the effects of oxymatrine on cell viability and apoptosis.
Statistical Information
P-Value
<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website