SARS-CoV Replication Supported by Modified Endoplasmic Reticulum Network
Author Information
Author(s): Knoops Kèvin, Kikkert Marjolein, van den Worm Sjoerd H. E., Zevenhoven-Dobbe Jessika C, van der Meer Yvonne, Koster Abraham J, Mommaas A. Mieke, Snijder Eric J
Primary Institution: Leiden University Medical Center
Hypothesis
How does SARS-CoV utilize host cell membranes for its replication?
Conclusion
SARS-CoV induces a complex network of modified endoplasmic reticulum membranes that supports its RNA synthesis.
Supporting Evidence
- SARS-CoV infection leads to the formation of double-membrane vesicles (DMVs) that are interconnected.
- The interior of DMVs labels abundantly for double-stranded RNA, indicating active viral replication.
- Electron tomography revealed that DMVs are part of a reticulovesicular network continuous with the rough endoplasmic reticulum.
Takeaway
SARS-CoV makes a special network inside infected cells to help it make more viruses and hide from the body's defenses.
Methodology
Electron tomography was used to visualize the membrane alterations in SARS-CoV-infected cells.
Limitations
The study could not definitively determine the connection between DMV interiors and the cytosol.
Digital Object Identifier (DOI)
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