High Throughput ELISAs for Measuring Glycans on Transferrin in Cerebrospinal Fluid
Author Information
Author(s): Keiro Shirotani, Satoshi Futakawa, Kiyomitsu Nara, Kyoka Hoshi, Toshie Saito, Yuriko Tohyama, Shinobu Kitazume, Tatsuhiko Yuasa, Masakazu Miyajima, Hajime Arai, Atsushi Kuno, Hisashi Narimatsu, Yasuhiro Hashimoto
Primary Institution: Fukushima Medical University School of Medicine
Hypothesis
Can high-throughput lectin-antibody ELISAs distinguish between different glycoforms of transferrin in cerebrospinal fluid for diagnosing idiopathic normal pressure hydrocephalus?
Conclusion
The study found that SSA-reactive transferrin levels were significantly higher in cerebrospinal fluid from patients with idiopathic normal pressure hydrocephalus compared to non-iNPH patients.
Supporting Evidence
- The lectin-antibody ELISAs can distinguish between CSF-type and serum-type transferrin.
- SSA-reactive transferrin levels were significantly higher in iNPH patients.
- The developed ELISAs are high throughput and suitable for clinical use.
- Previous methods like immunoblotting were impractical for clinical use due to low throughput.
- The study suggests potential for these ELISAs in diagnosing other neurological diseases.
Takeaway
The researchers created a new test to measure specific sugars on a protein in the brain fluid, which could help doctors tell if someone has a certain type of dementia.
Methodology
The study used high-throughput lectin-antibody ELISAs to measure different glycoforms of transferrin in cerebrospinal fluid samples from patients.
Limitations
The study focused only on idiopathic normal pressure hydrocephalus and did not explore other neurological diseases in depth.
Participant Demographics
28 iNPH patients (14 males, 14 females) aged 75.2 ± 6.1 years and 18 non-iNPH patients (10 males, 8 females) aged 74.9 ± 5.2 years.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website