Human macrophage response to the emerging enteric pathogen Aeromonas veronii: Inflammation, apoptosis, and downregulation of histones
2024

How Aeromonas veronii Affects Human Macrophages

publication Evidence: high

Author Information

Author(s): Nicholas Naidovski, Sarah K.T. Chong, Fang Liu, Stephen Riordan, Michael C. Wehrhahn, Christopher Yuwono, Li Zhang

Primary Institution: University of New South Wales

Hypothesis

This study investigates the pathogenic mechanisms of Aeromonas veronii in macrophages.

Conclusion

Aeromonas veronii induces higher levels of inflammation and apoptosis in human macrophages compared to E. coli.

Supporting Evidence

  • Aeromonas veronii significantly upregulated pro-inflammatory cytokines IL-8, IL-1β, IL-18, and TNFα.
  • Aeromonas veronii induced higher levels of apoptosis in THP-1 macrophages compared to E. coli.
  • Aeromonas veronii downregulated histone encoding genes, which may impair macrophage function.

Takeaway

Aeromonas veronii makes our immune cells very active and can even make them die, which might help it cause sickness.

Methodology

THP-1 derived macrophages were treated with A. veronii or E. coli, followed by RNA sequencing and protein level measurements of cytokines.

Statistical Information

P-Value

p<0.05

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1080/21505594.2024.2440554

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