PIAS1 interacts with FLASH and enhances its co-activation of c-Myb
2011
PIAS1 Enhances FLASH's Role in Activating c-Myb
publication
Evidence: moderate
Author Information
Author(s): Alm-Kristiansen Anne Hege, Lorenzo Petra I, Molværsmyr Ann-Kristin, Matre Vilborg, Ledsaak Marit, Sæther Thomas, Gabrielsen Odd S
Primary Institution: Department of Molecular Biosciences, University of Oslo
Hypothesis
Does PIAS1 interact with FLASH and enhance its co-activation of c-Myb?
Conclusion
PIAS1 is a common partner for FLASH and c-Myb, enhancing their transcriptional activity.
Supporting Evidence
- FLASH and PIAS1 co-localize in nuclear speckles.
- PIAS1 enhances the transcriptional activity of FLASH in a RING finger-dependent manner.
- PIAS1 and FLASH together enhance c-Myb dependent transcription.
Takeaway
PIAS1 helps FLASH work better with c-Myb, which is important for controlling gene activity in cells.
Methodology
Yeast two-hybrid screening, co-immunoprecipitation, and functional assays were used to study the interactions and effects of PIAS1 on FLASH and c-Myb.
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website