Mapping the Yeast Proteomic Environment
Author Information
Author(s): Anna Shevchenko, Assen Roguev, Daniel Schaft, Luke Buchanan, Bianca Habermann, Cagri Sakalar, Henrik Thomas, Nevan J Krogan, Andrej Shevchenko, A Francis Stewart
Primary Institution: MPI of Molecular Cell Biology and Genetics
Hypothesis
High resolution mapping of the proteomic environment and proteomic hyperlinks in fission and budding yeast reveals that divergent hyperlinks are due to gene duplications.
Conclusion
High resolution mapping of proteomic environments will deliver the highest quality data for comparative proteomics.
Supporting Evidence
- The Saccharomyces cerevisiae proteome presents the highest quality proteomic data available.
- Using sequential tagging, new subunits and complexes were uncovered.
- The architecture of the proteomic environment was found to be conserved between S. cerevisiae and S. pombe.
Takeaway
Scientists studied proteins in yeast to understand how they interact and work together, finding that some proteins are similar in different types of yeast.
Methodology
The study used tandem affinity purification and mass spectrometry to map protein complexes in yeast.
Limitations
The stringency of protein selection criteria may have excluded some lowly abundant interactors.
Digital Object Identifier (DOI)
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