The Effect of Phosphorylation on Peptide Ion Analysis
Author Information
Author(s): Creese Andrew J., Cooper Helen J.
Primary Institution: School of Biosciences, University of Birmingham, UK
Hypothesis
How does phosphorylation affect the electron capture dissociation of peptide ions?
Conclusion
Phosphorylation negatively impacts the sequence coverage of doubly-charged peptide ions during electron capture dissociation.
Supporting Evidence
- Phosphorylation reduces the sequence coverage of peptides during analysis.
- Increasing electron energy improves the detection of peptide fragments.
- Triply-charged precursor ions provide better sequence coverage than doubly-charged ones.
- Salt bridges formed by deprotonated phospho-groups hinder fragmentation.
- Different cathode potentials affect the efficiency of electron capture dissociation.
Takeaway
When proteins are modified by adding a phosphate group, it can make it harder to see all parts of the protein when using certain analysis methods.
Methodology
The study involved synthesizing various phosphopeptides and analyzing their electron capture dissociation behavior using mass spectrometry.
Limitations
The study primarily focused on synthetic peptides and may not fully represent the behavior of naturally occurring phosphopeptides.
Digital Object Identifier (DOI)
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