Transcriptional Regulation of Metabolic Fluxes in Yeast
Author Information
Author(s): Çakır Tunahan, Kırdar Betül, Önsan Z İlsen, Ülgen Kutlu Ö, Nielsen Jens
Primary Institution: Boğaziçi University
Hypothesis
How do changes in carbon sources affect the transcriptional regulation of metabolic fluxes in Saccharomyces cerevisiae?
Conclusion
Changes in carbon sources are associated with a high degree of hierarchical regulation of metabolic fluxes in the central carbon metabolism.
Supporting Evidence
- Changes in control-effective fluxes were correlated with transcript levels.
- 75% of the genes showed qualitative agreement with CEF changes.
- A regression correlation coefficient of 0.60 was achieved.
- Up-regulation of central metabolic genes was observed with carbon source shifts.
Takeaway
When yeast eats different sugars, it changes how it makes energy, and this study shows that these changes are closely linked to how the yeast's genes are turned on or off.
Methodology
The study used a metabolic model to analyze control-effective fluxes (CEF) and gene expression changes in response to different carbon sources.
Limitations
The correlation for amino acid metabolism was not established, and the study focused only on carbon source perturbations.
Statistical Information
P-Value
<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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