Complete Genome Sequence of Thermomicrobium roseum
Author Information
Author(s): Wu Dongying, Raymond Jason, Wu Martin, Chatterji Sourav, Ren Qinghu, Graham Joel E., Bryant Donald A., Robb Frank, Colman Albert, Tallon Luke J., Badger Jonathan H., Madupu Ramana, Ward Naomi L., Eisen Jonathan A.
Primary Institution: University of California Davis
Hypothesis
The study aims to enrich the phylogenetic diversity represented in sequenced bacterial genomes by determining the genome sequence of Thermomicrobium roseum.
Conclusion
The genome analysis of T. roseum reveals insights into its unique biology, including its ability to oxidize CO aerobically and the unusual composition of its cell membrane.
Supporting Evidence
- T. roseum is the first thermophile known to oxidize CO aerobically.
- The genome consists of two circular DNA elements, a chromosome and a megaplasmid.
- Phylogenomic analyses support the reclassification of T. roseum from the phylum Thermomicrobia to Chloroflexi.
- The cell membrane is composed entirely of long-chain 1,2-diols, which is unusual for bacteria.
- T. roseum encodes a complete system for chemotaxis on its megaplasmid.
Takeaway
Scientists sequenced the DNA of a special bacterium called T. roseum to learn more about how it lives in hot springs and how it might help us understand other bacteria.
Methodology
The genome was sequenced using shotgun libraries and analyzed for its composition and functional genes.
Limitations
The study primarily focuses on one strain and may not represent the full diversity of T. roseum in different environments.
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website