Modification of non-conservative double-strand break (DSB) rejoining activity after the induction of cisplatin resistance in human tumour cells
1999
Cisplatin Resistance and DNA Repair in Tumor Cells
publication
Author Information
Author(s): Britten R A, Kuny S, Perdue S
Primary Institution: Department of Oncology, University of Alberta, Cross Cancer Institute
Hypothesis
The study investigates the mechanisms behind cisplatin-induced radioresistance in human tumor cells.
Conclusion
Cisplatin resistance is linked to a significant decrease in non-conservative double-strand break rejoining activity in tumor cells.
Supporting Evidence
- The study found no significant difference in radiation-induced DNA double strand breaks between sensitive and resistant cell lines.
- There was a significantly lower level of DSB misrejoining activity in sensitive cell lines compared to resistant ones.
- DSB misrejoining events involved deletions that arose through illegitimate recombination.
Takeaway
When cancer cells become resistant to cisplatin, they also become less able to fix their DNA when it's damaged by radiation.
Statistical Information
Statistical Significance
p<0.01
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website