Absence of p300 induces cellular phenotypic changes characteristic of epithelial to mesenchyme transition
2006
p300 Deficiency and Epithelial to Mesenchyme Transition in Cancer Cells
publication
Evidence: moderate
Author Information
Author(s): Krubasik D, Iyer N G, English W R, Ahmed A A, Vias M, Roskelley C, Brenton J D, Caldas C, Murphy G
Primary Institution: University of Cambridge
Hypothesis
p300 deficiency induces EMT.
Conclusion
Loss of p300 in colorectal cancer cells leads to aggressive phenotypes characterized by reduced adhesion and increased migration.
Supporting Evidence
- p300− cells showed reduced expression of E-cadherin and increased expression of Vimentin.
- p300− cells had significant defects in cell–cell adhesion.
- Reintroduction of p300 could restore the aggregation phenotype in p300− cells.
- p300− cells demonstrated increased migration through collagen and matrigel.
Takeaway
When a protein called p300 is missing in certain cancer cells, they become more mobile and less sticky, which can make them spread more easily.
Methodology
The study used p300 deficient HCT116 cells to analyze changes in gene expression and cellular behavior.
Limitations
The study primarily focused on a single cell line and may not generalize to other cancer types.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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