Dietary Methionine Supplementation and Its Effects on Mice After Irradiation
Author Information
Author(s): Zhang Wei-Wei, Xiang Yang, Chen Li, Liu Shao-Ting, Lin Chuan-Chuan, Li Jiu-Xuan, Xiang Li-Xin, Chen Nan-Xi, Shi Dong-Ling, Zhang Yang-Yang, Wang Xue-Ying, Hu Lan-Yue, Chen Sai, Luo Ya, Tan Cheng-Ning, Xue Pei-Pei, Jiang Yang-Zhou, Li Sheng-Wen Calvin, Yang Zhen-Xing, Dai Ji-Gang, Li Zhong-Jun, Ran Qian
Primary Institution: Army Medical University, Chongqing, China
Hypothesis
Does dietary methionine supplementation enhance hematopoiesis in mice after irradiation?
Conclusion
Dietary methionine supplementation improves irradiation tolerance in mice and promotes bone marrow hematopoiesis.
Supporting Evidence
- A high methionine diet improved survival rates in irradiated mice.
- Bone marrow recovery was enhanced with increased dietary methionine.
- High methionine levels were associated with increased S100A4 expression in macrophages.
- Macrophage polarization was influenced by dietary methionine levels.
- Folate supplementation mitigated the effects of excess homocysteine from high methionine diets.
- Inflammatory factors in the bone marrow were reduced with high methionine intake.
- High methionine diets promoted hematopoietic stem cell proliferation.
- Macrophage depletion reduced survival rates in mice on high methionine diets.
Takeaway
Feeding mice more methionine helps them recover better after radiation exposure, especially in their blood-making cells.
Methodology
Mice were fed diets with varying methionine levels and subjected to irradiation; survival rates, body weight, and hematopoietic recovery were measured.
Potential Biases
Potential bias in dietary control and environmental factors affecting mouse health.
Limitations
The study used a single dose of irradiation which may not fully mimic human acute radiation syndrome.
Participant Demographics
8-week-old male C57BL/6 mice.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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