Imaging Neutrophil Elastase Activity in Mouse Lung Injury
Author Information
Author(s): Kossodo Sylvie, Zhang Jun, Groves Kevin, Cuneo Garry J., Handy Emma, Morin Jeff, Delaney Jeannine, Yared Wael, Rajopadhye Milind, Peterson Jeffrey D.
Primary Institution: PerkinElmer
Hypothesis
Can a specific imaging agent detect and quantify neutrophil elastase activity in vivo during acute lung injury?
Conclusion
The study successfully developed a novel imaging agent that can noninvasively quantify neutrophil elastase activity in a mouse model of acute lung injury.
Supporting Evidence
- Significantly higher fluorescent signal was quantified in mice with LPS/fMLP-induced ALI compared to healthy controls.
- Treatment with the specific inhibitor sivelestat significantly reduced lung signal in mice with ALI.
- NE680 was used in vivo to image and quantify NE activity associated with lung inflammation.
Takeaway
Researchers created a special agent that lights up when it finds a specific enzyme in the lungs of mice with lung injury, helping to see how bad the injury is.
Methodology
The study used a near-infrared fluorescent imaging agent to detect neutrophil elastase activity in a mouse model of acute lung injury, validated through various imaging and biochemical assays.
Limitations
The uneven distribution of inflammation due to intranasal instillation may affect the results.
Participant Demographics
Female retired breeder CD-1 mice, aged 12–16 weeks.
Statistical Information
P-Value
0.0032
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website