How MSK1 Affects SCF Expression in Inflammation
Author Information
Author(s): Reber Laurent, Vermeulen Linda, Haegeman Guy, Frossard Nelly
Primary Institution: Université Louis Pasteur-Strasbourg-I, Faculté de Pharmacie, Illkirch, France
Hypothesis
MSK1-mediated phosphorylation of NF-κB p65 at Ser276 is crucial for the expression of stem cell factor (SCF) in inflammatory conditions.
Conclusion
MSK1 controls the expression of SCF through the phosphorylation of NF-κB p65 at Ser276, which is essential for its binding to the SCF gene enhancer during inflammation.
Supporting Evidence
- MSK1 induces phosphorylation of NF-κB p65 at Ser276 upon IL-1β treatment.
- Inhibition of MSK1 or its upstream kinases abolishes SCF expression.
- Transfection with a kinase-dead MSK1 mutant reduces SCF expression significantly.
Takeaway
When our body gets inflamed, a protein called MSK1 helps another protein, NF-κB, to turn on a gene that makes a growth factor called SCF. This process is important for how our body responds to inflammation.
Methodology
The study used ChIP experiments, Western blotting, and transfection of fibroblasts to analyze the role of MSK1 in SCF expression.
Limitations
The study primarily focuses on human lung fibroblasts, which may limit the generalizability of the findings to other cell types or conditions.
Participant Demographics
Human lung fibroblasts from different donors were used in the experiments.
Digital Object Identifier (DOI)
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