Protein Changes in E. coli During Sugar Switching
Author Information
Author(s): Ekaterina Mostovenko, André M Deelder, Magnus Palmblad
Primary Institution: Leiden University Medical Center
Hypothesis
How does protein expression change in E. coli during the glucose-lactose diauxie?
Conclusion
The study confirmed that β-galactosidase is the most strongly induced protein during the glucose-lactose shift in E. coli.
Supporting Evidence
- The onset of diauxie occurred at a cell density of approximately 5 × 10^8 cells/mL.
- 4,333 peptides from 948 proteins were identified during the study.
- β-galactosidase expression increased almost 16-fold during the diauxic shift.
Takeaway
When E. coli switches from using glucose to lactose, it makes a lot more of a protein called β-galactosidase to help it digest the lactose.
Methodology
E. coli was grown in a medium with glucose and lactose, and protein expression was analyzed using label-free quantitative mass spectrometry.
Limitations
The study's protein expression measurements were not always in perfect agreement with published gene expression data.
Digital Object Identifier (DOI)
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