Setdb1 and Atf7IP form a hetero-trimeric complex that blocks Setdb1 nuclear export
2024
Setdb1 and Atf7IP: A Complex That Keeps Setdb1 in the Nucleus
publication
Author Information
Author(s): Kariapper Leena, Marathe Ila A., Niesman Ashley B., Suino-Powell Kelly, Chook Yuh Min, Wysocki Vicki H., Worden Evan J.
Primary Institution: Cold Spring Harbor Laboratory
Hypothesis
The interaction between Setdb1 and Atf7IP is crucial for Setdb1's nuclear localization and function.
Conclusion
Setdb1 and Atf7IP form a complex that prevents Setdb1 from being exported out of the nucleus.
Supporting Evidence
- Setdb1 and Atf7IP form a hetero-trimeric complex in vitro and in cells.
- Atf7IP self-associates to form multimeric complexes.
- Setdb1 binds to Atf7IP through coiled coil interactions.
- Atf7IP competes with CRM1 to bind Setdb1's nuclear export signals.
Takeaway
Setdb1 and Atf7IP work together to keep Setdb1 inside the cell's nucleus, which helps control certain genes.
Methodology
The study used Alphafold2 predictions and biochemical reconstitutions to analyze the Setdb1 and Atf7IP interaction.
Digital Object Identifier (DOI)
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