NADPH Oxidase Subunit 4-Mediated Reactive Oxygen Species Contribute to Cycling Hypoxia-Promoted Tumor Progression in Glioblastoma Multiforme
2011

NADPH Oxidase Subunit 4 and Tumor Progression in Glioblastoma

Sample size: 6 publication 10 minutes Evidence: high

Author Information

Author(s): Hsieh Chia-Hung, Shyu Woei-Cherng, Chiang Chien-Yi, Kuo Jung-Wen, Shen Wu-Chung, Liu Ren-Shyan

Primary Institution: China Medical University and Hospital

Hypothesis

Nox4 is a critical mediator of cycling hypoxia-mediated ROS generation and tumor progression in glioblastoma.

Conclusion

Cycling hypoxia-induced ROS via Nox4 is a critical aspect of cancer biology to consider for therapeutic targeting of HIF-1 activation and tumor progression in GBM.

Supporting Evidence

  • Cycling hypoxia increased ROS production and HIF-1 activation in glioblastoma cells.
  • Nox4 knockdown inhibited cycling hypoxia-induced effects.
  • Tempol treatment reduced ROS levels and tumor growth in vivo.

Takeaway

This study found that low oxygen levels in tumors can make them grow faster by producing harmful molecules, and blocking these molecules could help treat brain cancer.

Methodology

Glioblastoma cell lines were exposed to cycling hypoxic stress in vitro and in vivo, with ROS production measured through various assays.

Statistical Information

P-Value

p<0.01

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1371/journal.pone.0023945

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