High Resolution BAC Contig on Bovine Chromosome 1q12
Author Information
Author(s): Cord Drogemuller, Anne Wohlke, Tosso Leeb, Ottmar Distl
Primary Institution: Institute for Animal Breeding and Genetics, University of Veterinary Medicine, Hannover, Germany
Hypothesis
The study aims to construct a high-resolution BAC contig on bovine chromosome 1q12 and compare it with human chromosome 21q22.
Conclusion
The constructed BAC contig provides a valuable resource for the positional cloning of the polled gene in cattle.
Supporting Evidence
- The BAC contig covers the region between the genes KRTAP8P1 and CLIC6.
- 31 orthologues of human genes located on HSA 21q22 were directly mapped within the bovine BAC contig.
- The mapping of known polled linked BTA 1q12 microsatellite markers allowed the integration of the physical contig map with existing linkage maps.
Takeaway
Scientists built a map of a part of cow DNA to help find the gene that makes some cows hornless.
Methodology
The study involved screening a bovine BAC library, constructing a contig of BAC clones, and performing comparative mapping with human genes.
Limitations
The recombination frequency could not be reliably estimated due to inconsistencies between different genetic maps.
Digital Object Identifier (DOI)
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