Bone Marrow Mononuclear Cells Up-Regulate Toll-Like Receptor Expression and Produce Inflammatory Mediators in Response to Cigarette Smoke Extract
2011

Cigarette Smoke Extract Affects Bone Marrow Cells

Sample size: 3 publication 10 minutes Evidence: high

Author Information

Author(s): Zhou Junmin, Eksioglu Erika A., Fortenbery Nicole R., Chen Xianghong, Wang Huaquan, Epling-Burnette Pearlie K., Djeu Julie Y., Wei Sheng

Primary Institution: H. Lee Moffitt Cancer Center and Research Institute

Hypothesis

What are the direct effects of cigarette smoke extract on human bone marrow hematopoiesis?

Conclusion

Cigarette smoke extract significantly inhibits the growth of certain bone marrow progenitor cells and induces inflammatory responses.

Supporting Evidence

  • Cigarette smoke extract reduced the colony formation of bone marrow cells.
  • Cigarette smoke extract induced nuclear translocation of NF-κB and activation of signaling pathways.
  • Cigarette smoke extract increased the expression of Toll-like receptors in bone marrow cells.

Takeaway

Cigarette smoke can hurt the cells in our bone marrow that help make blood, which might lead to health problems like cancer.

Methodology

Bone marrow mononuclear cells from healthy individuals were exposed to different concentrations of cigarette smoke extract and analyzed for colony formation and cytokine production.

Limitations

The study was performed in vitro and may not fully replicate the complex effects of long-term cigarette smoking in vivo.

Participant Demographics

Healthy individuals

Statistical Information

P-Value

p<0.001

Statistical Significance

p<0.001

Digital Object Identifier (DOI)

10.1371/journal.pone.0021173

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