Role of Aspartate 42 and Histidine 79 in the aiPLA2 activity and oligomeric status of Prdx6 at low pH
2024
Role of Aspartate 42 and Histidine 79 in Prdx6 Activity at Low pH
publication
Author Information
Author(s): Kakchingtabam Pushpa, Shahnaj Sharifun, Kumari Anju, Longjam Japani, N. Wungnaopam A., Herojit Khundrakpam, Laishram Rajendrakumar Singh, Fisher Aron B., Rahaman Hamidur
Hypothesis
How do Asp42 and His79 influence the oligomeric state of Prdx6 at low pH?
Conclusion
Asp42 and His79 are crucial for the oligomerization of Prdx6 at low pH.
Supporting Evidence
- Prdx6 forms higher oligomers at low pH, enhancing its aiPLA2 activity.
- Mutations at Asp42 and His79 did not significantly change peroxidase activity at neutral pH.
- DLS measurements showed that wild-type Prdx6 forms oligomers at low pH, unlike the mutant proteins.
Takeaway
The study found that two specific parts of a protein help it change shape and stick together when the environment is acidic.
Methodology
Site-directed mutagenesis was used to study the effects of mutations on Prdx6 activity and oligomerization.
Digital Object Identifier (DOI)
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