Caspase-3 activation during apoptosis caused by glutathione–doxorubicin conjugate
1999
Caspase-3 Activation in Cancer Cell Death
publication
Evidence: moderate
Author Information
Author(s): Asakura T, Sawai T, Hashidume Y, Ohkawa Y, Yokoyama S, Ohkawa K
Primary Institution: Jikei University School of Medicine
Hypothesis
Does glutathione–doxorubicin conjugate induce apoptosis more effectively than doxorubicin alone?
Conclusion
Glutathione–doxorubicin induces apoptosis in rat hepatoma cells more effectively than doxorubicin alone by activating caspase-3.
Supporting Evidence
- Glutathione–doxorubicin induced apoptosis at lower concentrations than doxorubicin.
- Caspase-3 activity increased in a dose-dependent manner after treatment.
- DNA fragmentation was observed after 24 hours of drug treatment.
Takeaway
This study shows that a special drug combination can make cancer cells die better than just one drug alone.
Methodology
The study involved treating rat hepatoma cells with glutathione–doxorubicin and measuring caspase-3 activity and DNA fragmentation.
Participant Demographics
Rat hepatoma cells (AH66)
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website