Changes of PAF and TNF in myocardial ischaemia and reperfusion injury
Author Information
Author(s): Qin-Yue, Shao-Ha Li, Ya-Ling Han, Zheng, Hong-Bin Wang, Guo-Yan Zhang, Zhong-Gui Wu, Si-Cong Chen
Primary Institution: Department of Cardiology, Chang Zheng Hospital, Shanghai, China; Department of Pathophysiology and Pharmacology (School of Pharmacy), Second Military Medical University, Shanghai, China
Hypothesis
The study investigates the role and interaction between platelet-activating factor (PAF) and tumor necrosis factor (TNF) in myocardial ischaemia and reperfusion injury.
Conclusion
The study concludes that PAF and TNF are important mediators in myocardial ischaemia and reperfusion injury, and that the PAF antagonist BN 50739 can reduce infarct size.
Supporting Evidence
- Blood PAF levels significantly increased after myocardial ischaemia and further increased after reperfusion.
- Serum TNF levels also increased significantly during ischaemia and reperfusion.
- BN 50739 significantly reduced infarct size in treated groups compared to vehicle controls.
Takeaway
This study found that two substances in the blood, PAF and TNF, can cause heart damage when blood flow is blocked and then restored, but a special medicine can help protect the heart.
Methodology
The study used an anaesthetized rat model with coronary artery ligation and reperfusion to measure blood PAF and serum TNF levels, and the effects of the PAF antagonist BN 50739 on infarct size.
Participant Demographics
Sprague-Dawley male rats, weighing between 260 and 330 g.
Statistical Information
P-Value
p<0.05, p<0.01
Statistical Significance
p<0.05
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