CrossHybDetector: A Tool for Detecting Cross-Hybridization in DNA Microarrays
Author Information
Author(s): Paolo Uva, Emanuele de Rinaldis
Primary Institution: Istituto di Ricerche di Biologia Molecolare, Merck Research Laboratories
Hypothesis
Can CrossHybDetector effectively identify cross-hybridization events in DNA microarray experiments?
Conclusion
CrossHybDetector is a software package that successfully identifies cross-hybridization events in DNA microarray experiments.
Supporting Evidence
- The software identified 277 corrupted probes in the Phage TAG array and 940 in the Yeast TAG array.
- The average Pearson's correlation for corruptor/corrupted pairs was significantly higher than for random pairs.
- CrossHybDetector was able to reduce the number of hybridization experiments affected by cross-hybridization from 22 to 5.
Takeaway
CrossHybDetector is a computer program that helps scientists find mistakes in DNA tests caused by similar sequences sticking together.
Methodology
The software uses probe sequences and array intensity values to identify cross-hybridization events through Monte Carlo simulations.
Limitations
CrossHybDetector cannot detect cross-hybridization events that do not cause an intensity increase in the spots carrying similar probe sequences.
Statistical Information
P-Value
<0.0001
Statistical Significance
p<0.0001
Digital Object Identifier (DOI)
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