How Pseudomonas aeruginosa Uses a Pump to Export a Signaling Molecule
Author Information
Author(s): Lamarche Martin G., Déziel Eric
Primary Institution: INRS-Institut Armand-Frappier, Laval, Québec, Canada
Hypothesis
The MexEF-OprN efflux pump modulates quorum sensing through secretion of a signaling molecule belonging to the HAQ family.
Conclusion
The study shows that the MexEF-OprN efflux pump exports HHQ, a precursor of PQS, which affects the virulence of Pseudomonas aeruginosa.
Supporting Evidence
- The MexEF-OprN efflux pump was shown to specifically modulate the production of HHQ and PQS.
- Mutants overexpressing MexEF-OprN produced higher levels of HHQ but lower levels of PQS.
- Efflux pump inhibitors increased the intracellular concentration of HHQ and restored PQS production.
- Knocking out the mexE gene in a mexS mutant restored QS-related virulence phenotypes.
Takeaway
Bacteria can talk to each other using tiny signals, and this study found that a specific pump in Pseudomonas aeruginosa helps it send out one of those signals, which can make it more harmful.
Methodology
The study used various Pseudomonas aeruginosa mutants to analyze the effects of the MexEF-OprN efflux pump on quorum sensing and virulence factor production.
Limitations
The specific mechanisms behind the observed effects of MexEF-OprN on quorum sensing and virulence factors remain unclear.
Digital Object Identifier (DOI)
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