Comparing Proteomic Profiles of Human Glomeruli from Small Samples
Author Information
Author(s): Zhang Ying, Yoshida Yutaka, Xu Bo, Magdeldin Sameh, Fujinaka Hidehiko, Liu Zan, Miyamoto Masahito, Yaoita Eishin, Yamamoto Tadashi
Primary Institution: Niigata University, Niigata, Japan
Hypothesis
Can high-confidence proteomic analysis be performed on small amounts of human glomeruli comparable to biopsy samples using different mass spectrometry techniques?
Conclusion
The study demonstrated that representative human glomerulus proteomic profiles can be obtained from small samples using various mass spectrometry methods.
Supporting Evidence
- The study identified 332 distinct glomerular proteins from small samples.
- Around 60% of the proteins were detected by more than two mass spectrometry platforms.
- 14 proteins were uniquely identified in this study compared to a comprehensive database.
Takeaway
Researchers found that they could identify important proteins in tiny samples of kidney tissue, which helps in understanding kidney diseases better.
Methodology
The study analyzed one microgram of human glomerular protein digest on five different mass spectrometers.
Limitations
The study was limited by the small amount of tissue available for analysis.
Participant Demographics
The kidney tissue was obtained from a 49-year-old male patient.
Statistical Information
P-Value
p<0.01
Statistical Significance
p<0.01
Digital Object Identifier (DOI)
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