Reversible suppression by nalidixic acid of anchorage-independent growth of mouse cells transformed by 3-methylcholanthrene or an activated c-Ha-ras gene
1989
Nalidixic Acid Suppresses Growth of Transformed Mouse Cells
publication
Evidence: moderate
Author Information
Author(s): M. Kaneko, J. Horikoshi
Primary Institution: National Cancer Centre Research Institute, Biophysics Division, Tokyo, Japan
Hypothesis
Are Nal and its derivative effective in suppressing anchorage-independent growth of transformed Balb/3T3 mouse cells induced by MC?
Conclusion
Nalidixic acid reversibly suppresses the expression of transformed phenotypes in mouse cells induced by either 3-methylcholanthrene or an activated c-Ha-ras gene.
Supporting Evidence
- Nalidixic acid suppressed growth in soft agar of transformed Balb/3T3 mouse cells induced by methylcholanthrene.
- The suppressive effect of nalidixic acid on growth in soft agar was reversible.
- Nalidixic acid did not affect the amount of ras p21 proteins in NIH/3T3 mouse cells transformed by an activated c-Ha-ras.
Takeaway
Nalidixic acid can stop certain cancer cells from growing in a special way, but this effect goes away if the drug is removed.
Methodology
The study involved culturing transformed mouse cells in soft agar and assessing the effects of nalidixic acid on their growth.
Want to read the original?
Access the complete publication on the publisher's website