Structure of the met protein and variation of met protein kinase activity among human tumour cell lines
1988
Variation of Met Protein Kinase Activity in Human Tumor Cell Lines
publication
Evidence: moderate
Author Information
Author(s): P.R. Tempest, M.R. Stratton, C.S. Cooper
Primary Institution: Institute of Cancer Research
Hypothesis
Can variations in met protein kinase activity among human tumor cell lines be linked to tumor transformation?
Conclusion
The study found significant variations in met protein kinase activity among different human tumor cell lines.
Supporting Evidence
- High levels of autophosphorylation were observed in A431 cervical carcinoma cells.
- Daoy medulloblastoma cells showed no phosphorylation of the 140 kD met protein but high phosphorylation of a 132 kD protein.
- The met protein may exist as a complex of a 140 kD beta subunit and a 25 kD alpha subunit.
Takeaway
Some cancer cells have different amounts of a protein that helps them grow. This study looked at how much of this protein is in different types of cancer cells.
Methodology
The study used an in vitro autophosphorylation assay and SDS-polyacrylamide gel electrophoresis to analyze met protein kinase activity and structure.
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