NF-κB Inhibition and Tumor Cell Radiosensitivity
Author Information
Author(s): Ridder M De, Van den Berge D L, Verovski V N, Monsaert C, Wauters N, Storme G A
Primary Institution: Academic Hospital Free University Brussels (A.Z.-V.U.B.)
Hypothesis
Inhibition of NF-κB might impair rather than enhance the radiosensitivity of hypoxic tumour cells through transcriptional downregulation of iNOS.
Conclusion
Inhibition of NF-κB decreases the radiosensitivity of hypoxic EMT-6 tumour cells by downregulating iNOS.
Supporting Evidence
- Blocking NF-κB reduced the radiosensitivity of hypoxic EMT-6 cells.
- iNOS expression was significantly higher in hypoxia compared to normoxia.
- Both lactacystin and PAO inhibited the expression of iNOS.
Takeaway
This study found that blocking a certain protein (NF-κB) in cancer cells makes them less sensitive to radiation, especially when there's low oxygen.
Methodology
EMT-6 tumour cells were exposed to LPS in hypoxic conditions, and the effects on NF-κB activation, iNOS induction, and radiosensitivity were evaluated.
Statistical Information
P-Value
p<0.001
Statistical Significance
p<0.001
Digital Object Identifier (DOI)
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