Protective Effects of Ocimum sanctum Against Chlorpyrifos-Induced Damage
Author Information
Author(s): Khanna Asha, Shukla Poonam, Tabassum Shajiya
Primary Institution: Department of Zoology and Biotechnology, St. Aloysius’ College, Pachpedi, Madhya Pradesh, India
Hypothesis
Does Ocimum sanctum have a genoprotective effect against chlorpyrifos-induced genotoxicity?
Conclusion
Ocimum sanctum significantly reduces the genotoxic effects of chlorpyrifos in both in vivo and in vitro models.
Supporting Evidence
- Rats pretreated with Ocimum sanctum showed a significant increase in mitotic index compared to those treated with chlorpyrifos alone.
- Chromosomal aberrations were significantly reduced in cultures pretreated with Ocimum sanctum extract.
- DNA damage was assessed using comet assay, showing that chlorpyrifos caused significant damage that was mitigated by Ocimum sanctum.
Takeaway
This study shows that a plant called Tulsi can help protect cells from damage caused by a pesticide.
Methodology
The study used in vivo rat models and in vitro human lymphocyte cultures to assess the protective effects of Ocimum sanctum against chlorpyrifos-induced genotoxicity.
Limitations
The study primarily focused on specific concentrations of chlorpyrifos and may not represent all exposure scenarios.
Participant Demographics
Albino rats and human lymphocyte cultures were used in the study.
Statistical Information
P-Value
p<0.001 for MI and p<0.05 for CA
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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