How PDGF Affects Cell Growth in Eye Cells
Author Information
Author(s): Chen Kate, Chao-Wei Zhou, Wei Zhang, Marjorie F. Lou
Primary Institution: University of Nebraska-Lincoln
Hypothesis
The study investigates the mechanism of PDGF-induced reactive oxygen species (ROS) generation and its role in cell proliferation in human lens epithelial cells.
Conclusion
The study clarifies that PDGF signaling in lens epithelial cells requires the cooperation of several upstream components, including PDGF receptor kinase, Src-family kinases, PI3K, Rac, and Ras proteins.
Supporting Evidence
- Inhibiting PDGF receptor kinase completely abolished ROS production and cell growth.
- Co-inhibiting PDGF and EGF receptors eradicated the PDGF signaling system.
- Transiently transfected cells with dominant negative Rac or Ras diminished PDGF action in ROS generation and cell proliferation.
Takeaway
When a special protein called PDGF is present, it helps eye cells grow by creating tiny molecules called ROS. These molecules help send signals that make the cells multiply.
Methodology
The study used confocal microscopy to measure ROS generation and Western blot analysis to determine activated MAP kinases in human lens epithelial cells.
Statistical Information
P-Value
<0.001
Statistical Significance
p<0.001
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