WIP Regulates the Stability and Localization of WASP to Podosomes in Migrating Dendritic Cells
2006

WIP Regulates WASP in Dendritic Cells

Sample size: 80 publication Evidence: high

Author Information

Author(s): Chou Hsiu-Chuan, Antón Inés M., Holt Mark R., Curcio Claudia, Lanzardo Stefania, Worth Austen, Burns Siobhan, Thrasher Adrian J., Jones Gareth E., Calle Yolanda

Primary Institution: King's College London

Hypothesis

What role does WIP play in the regulation of podosome architecture and cell motility in dendritic cells?

Conclusion

WIP is essential for podosome formation and cell polarity in dendritic cells by preventing WASP degradation and facilitating its localization.

Supporting Evidence

  • WIP is essential for the formation of actin cores containing WASP and cortactin.
  • WIP regulates the turnover of adhesions and polarity in dendritic cells.
  • WIP prevents WASP degradation by calpain.

Takeaway

WIP helps a protein called WASP to stay stable and in the right place so that immune cells can move and stick properly.

Methodology

The study used confocal microscopy to examine the distribution of F-actin and vinculin in wild-type and WIP−/− dendritic cells.

Participant Demographics

Dendritic cells from wild-type and WIP−/− mice were used.

Statistical Information

P-Value

p<0.05

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1016/j.cub.2006.10.037

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