Resistin's Role in Insulin Sensitivity and Inflammation in Adipocytes
Author Information
Author(s): Fu Yuchang, Luo Liehong, Luo Nanlan, Garvey W Timothy
Primary Institution: University of Alabama at Birmingham
Hypothesis
Does overexpression of resistin in adipocytes affect insulin sensitivity and proinflammatory cytokine production?
Conclusion
Overexpression of resistin in adipocytes decreases insulin responsiveness and increases proinflammatory cytokine production.
Supporting Evidence
- Resistin overexpression decreased GLUT4 expression by 51%.
- Proinflammatory cytokines TNFα, IL-6, and MCP-1 increased by 10-, 3-, and 21-fold respectively with resistin overexpression.
- Insulin-stimulated glucose transport activity decreased by 30% in resistin-overexpressing adipocytes.
Takeaway
When fat cells make too much of a protein called resistin, they have a harder time using sugar and make more substances that cause inflammation.
Methodology
The study involved stably transducing 3T3-L1 fibroblasts with a lentiviral vector to overexpress resistin and measuring its effects on insulin sensitivity and cytokine production.
Limitations
The study was conducted in vitro, which may not fully replicate in vivo conditions.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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