The pervasive effects of an antibiotic on the human gut microbiota, as revealed by deep 16S rRNA sequencing
2008

Effects of Ciprofloxacin on Human Gut Microbiota

Sample size: 3 publication 10 minutes Evidence: moderate

Author Information

Author(s): Dethlefsen L, Huse S, Sogin ML, Relman DA

Primary Institution: Stanford University

Hypothesis

How does ciprofloxacin treatment affect the composition and diversity of the human gut microbiota?

Conclusion

Ciprofloxacin treatment significantly alters the gut microbiota, reducing diversity and richness, but most taxa return to baseline levels within four weeks.

Supporting Evidence

  • Ciprofloxacin treatment influenced the abundance of about a third of the bacterial taxa in the gut.
  • Taxonomic composition returned to pretreatment state by four weeks after treatment.
  • Some taxa failed to recover within six months.

Takeaway

Taking the antibiotic ciprofloxacin changes the bacteria in your gut, but most of them come back to normal after a month.

Methodology

The study used deep 16S rRNA sequencing to analyze stool samples from three healthy adults before, during, and after ciprofloxacin treatment.

Potential Biases

Potential biases may arise from individual differences in gut microbiota composition and antibiotic response.

Limitations

The study involved only three participants, which may limit the generalizability of the findings.

Participant Demographics

Three healthy adults who had not taken antibiotics in the previous year.

Statistical Information

P-Value

p<0.005

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1371/journal.pbio.0060280

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