Effects of Lysosomal Membrane Protein Depletion on the Salmonella-Containing Vacuole
2008
Effects of Lysosomal Membrane Protein Depletion on Salmonella Vacuole
publication
Evidence: moderate
Author Information
Author(s): Roark Everett A., Haldar Kasturi
Primary Institution: Northwestern University
Hypothesis
SifA is required for the recruitment of LAMP-2 to the Salmonella-containing vacuole.
Conclusion
LAMP-2 contributes to the stability of the Salmonella-containing vacuolar membrane.
Supporting Evidence
- LAMP-2 is recruited to the Salmonella-containing vacuole in infected cells.
- LAMP-2 recruitment is reduced in the absence of SifA.
- LAMP-2 knockdown promotes reduction of LAMP-1 at the SCV membrane.
Takeaway
This study shows that LAMP-2 helps keep Salmonella inside cells safe, while LAMP-1 is not as crucial.
Methodology
HeLa cells were infected with Salmonella and immuno-stained to detect LAMP-1 and LAMP-2.
Statistical Information
P-Value
0.0001
Statistical Significance
p<0.001
Digital Object Identifier (DOI)
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