Identification of a subunit of NADH-dehydrogenase as a p49/STRAP-binding protein
2008
Identifying a Protein That Binds to p49/STRAP
Sample size: 4
publication
Evidence: moderate
Author Information
Author(s): Zhang Xiaomin, Azhar Gohar, Helms Scott, Zhong Ying, Wei Jeanne Y
Primary Institution: The University of Arkansas for Medical Sciences
Hypothesis
Does p49/STRAP interact with NDUFAB1 and affect cellular metabolism?
Conclusion
The study suggests that p49/STRAP regulates the NAD/NADH ratio and influences gene expression and aging.
Supporting Evidence
- NDUFAB1 was found to be the main protein interacting with p49/STRAP in multiple screenings.
- Overexpression of p49/STRAP reduced the NAD/NADH ratio by approximately 40%.
- The study indicates that p49/STRAP may influence gene expression and aging.
Takeaway
Researchers found that a protein called p49/STRAP interacts with another protein, NDUFAB1, which helps control energy in cells. When p49/STRAP is more active, it changes how much energy the cells have.
Methodology
Yeast two-hybrid screening was used to identify proteins interacting with p49/STRAP, followed by co-immunoprecipitation and Western blotting.
Statistical Information
P-Value
<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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