How Butyrate Affects Liver Glycogen and ATP Production in Rats
Author Information
Author(s): Gallis Jean-Louis, Gin Henri, Roumes Hélène, Beauvieux Marie-Christine
Primary Institution: Centre de Résonance Magnétique des Systèmes Biologiques, Université Bordeaux Segalen, CNRS, LabEx TRAIL-IBIO
Hypothesis
Butyrate disturbs the energy supply necessary for glycogen synthesis in the liver.
Conclusion
Butyrate delays the transient increase in mitochondrial ATP turnover, which is linked to glycogen metabolism in the liver.
Supporting Evidence
- Butyrate delayed the increase in mitochondrial ATP turnover until the 6th hour post-feeding.
- A linear correlation between total ATP and glycogen contents was observed only during net glycogen synthesis.
- Net glycogen synthesis occurred until the 6th hour in the glucose group and the 8th hour in the butyrate group.
- Mitochondrial ATP turnover was stable during the first two hours regardless of the feeding type.
- Butyrate is known to reduce oxidative phosphorylation yield and induce a glucose-sparing effect.
Takeaway
This study shows that butyrate can slow down how quickly the liver makes energy and stores sugar after eating.
Methodology
Rats were fasted for 48 hours and then force-fed with glucose or glucose plus butyrate, with liver samples analyzed using NMR.
Potential Biases
Potential bias in the interpretation of NMR data and the effects of butyrate on metabolism.
Limitations
The study was conducted only in male Wistar rats, which may limit the generalizability of the findings.
Participant Demographics
Male Wistar rats weighing 90-120 g.
Statistical Information
P-Value
0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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