Nek6 regulates autophagy through the mTOR signaling pathway to alleviate cerebral ischemia–reperfusion injury
2024
Nek6 and Its Role in Reducing Brain Injury After Stroke
Sample size: 20
publication
10 minutes
Evidence: high
Author Information
Author(s): Wang Qingzhi, Liu Xinjing, Yuan Jing, Yang Ting, Ding Lan, Song Bo, Xu Yuming
Primary Institution: The First Affiliated Hospital of Zhengzhou University
Hypothesis
Nek6 alleviates cerebral ischemia-reperfusion injury through autophagy regulation via the mTOR signaling pathway.
Conclusion
Nek6 regulates autophagy and alleviates cerebral ischemia-reperfusion injury through the mTOR signaling pathway.
Supporting Evidence
- Nek6 expression was increased in both MCAO and OGD/R models.
- Overexpression of Nek6 inhibited apoptosis and decreased autophagy-related protein expression.
- Nek6 overexpression promoted p-Akt and p-mTOR protein expressions.
- Interference with Nek6 had opposite effects on cell apoptosis.
- Abnormal METTL3 expression enhanced m6A modification and promoted Nek6 expression.
Takeaway
Nek6 helps protect the brain after a stroke by managing a process called autophagy, which is like cleaning up damaged cells.
Methodology
The study used mouse models of cerebral ischemia-reperfusion injury and various assays to measure cell apoptosis and autophagy.
Participant Demographics
C57BL/6 J male mice, aged 8-10 weeks.
Statistical Information
P-Value
p<0.01
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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