Nicotinamide, Fluosol DA and Carbogen: A strategy to reoxygenate acutely and chronically hypoxic cells in vivo
1991

Nicotinamide and Fluosol DA/Carbogen for Tumor Reoxygenation

Sample size: 6 publication Evidence: moderate

Author Information

Author(s): D.J. Chaplin, M.R. Horsman, D.S. Aoki

Primary Institution: B.C. Cancer Research Centre

Hypothesis

Can a combination of Nicotinamide and Fluosol DA/Carbogen effectively reoxygenate hypoxic tumor cells?

Conclusion

The combination of Nicotinamide and Fluosol DA/Carbogen significantly enhances the radiation response of both acutely and chronically hypoxic tumor cells.

Supporting Evidence

  • Nicotinamide significantly reduces acute hypoxia in tumors.
  • Combination treatment resulted in an enhancement factor of 1.6.
  • Fluosol DA/Carbogen alone did not significantly enhance radiation response.

Takeaway

This study shows that using Nicotinamide with Fluosol DA and Carbogen can help tumors get more oxygen, making them more sensitive to radiation treatment.

Methodology

Mice with SCCVII or KHT tumors were treated with Nicotinamide and/or Fluosol DA/Carbogen before radiation, and tumor cell survival was assessed using clonogenic assays.

Limitations

The enhancement of radiation response from Fluosol DA/Carbogen did not reach statistical significance in either tumor model.

Participant Demographics

6-9 week old female C3H/He mice

Statistical Information

P-Value

p<0.05

Statistical Significance

p<0.05

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