Detecting Papillomavirus in Snow Leopards
Author Information
Author(s): Katherine Mitsouras, Erica A Faulhaber, Gordon Hui, Janis O Joslin, Curtis Eng, Margaret C Barr, Kristopher J L Irizarry
Primary Institution: Western University of Health Sciences
Hypothesis
Can a PCR assay be developed to detect papillomavirus infection in snow leopards?
Conclusion
A PCR assay was successfully developed to detect papillomavirus in snow leopards using saliva samples.
Supporting Evidence
- The PCR assay specifically amplified viral DNA from saliva samples.
- Viral DNA was not detected in paired blood samples.
- The sequences of the PCR products were 100% identical to the published UuPV1 genome sequence.
Takeaway
Scientists created a test to find a virus in snow leopards' spit, which helps check if they are sick without hurting them.
Methodology
PCR amplification of E6 and E7 genes from saliva and blood samples of snow leopards.
Limitations
The study was limited to a small sample size and only included captive snow leopards.
Participant Demographics
Three captive snow leopards: two adult females (approximately 18 years old) and one 7-week old female cub.
Digital Object Identifier (DOI)
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