Effects of High Fat Diet on Inbred Mice
Author Information
Author(s): Jane F. Fearnside, Marc-Emmanuel Dumas, Alice R. Rothwell, Steven P. Wilder, Olivier Cloarec, Ayo Toye, Christine Blancher, Elaine Holmes, Roger Tatoud, Richard H. Barton, James Scott, Jeremy K. Nicholson, Dominique Gauguier
Primary Institution: The Wellcome Trust Centre for Human Genetics, University of Oxford
Hypothesis
Dietary changes including high fat diet feeding promotes insulin resistance in rodent models.
Conclusion
The study shows that different inbred mouse strains exhibit varying metabolic and hormonal responses to high fat diet feeding, influencing their susceptibility to insulin resistance and obesity.
Supporting Evidence
- Different mouse strains showed varied responses to high fat diets, indicating genetic predispositions to obesity.
- 129S6 and C57BL/6 strains were more susceptible to insulin resistance compared to BALB/c and DBA/2 strains.
- Metabonomic profiling revealed strain-specific metabolic changes in response to dietary fat.
- Hierarchical clustering of metabolic data matched genetic relationships between the strains.
Takeaway
This study looked at how different types of mice react to a high fat diet, finding that some mice get fatter and have more trouble with sugar than others.
Methodology
The study used a combination of physiological, biochemical, and hormonal studies along with plasma NMR spectroscopy to analyze the effects of a high fat diet on five inbred mouse strains.
Potential Biases
Potential bias in strain selection and environmental factors affecting metabolic responses.
Limitations
The study primarily focuses on short-term effects and may not capture long-term adaptations to high fat diets.
Participant Demographics
Male mice of five inbred strains: C57BL/6, 129S6, BALB/c, DBA/2, and C3H.
Statistical Information
P-Value
p<0.001
Statistical Significance
p<0.001
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website