NAGNAG Alternative Splicing in Moss
Author Information
Author(s): Rileen Sinha, Andreas D. Zimmer, Kathrin Bolte, Daniel Lang, Ralf Reski, Matthias Platzer, Stefan A. Rensing, Rolf Backofen
Primary Institution: University of Freiburg
Hypothesis
Is NAGNAG alternative splicing common in the moss Physcomitrella patens?
Conclusion
NAGNAG alternative splicing is common in the moss P. patens and can be predicted with high accuracy.
Supporting Evidence
- 295 alternatively used NAGNAG acceptors were identified using Sanger data.
- A classifier achieved a balanced specificity and sensitivity of ≥ 89%.
- 64% of the well-supported cases of AS were predicted correctly using additional transcript evidence.
Takeaway
This study found that a type of gene splicing called NAGNAG happens a lot in a moss plant, just like in other plants and animals.
Methodology
The study used Sanger sequencing and next-generation sequencing to identify and validate NAGNAG splicing events.
Limitations
The study's findings may not be generalizable to all plant species due to the specific focus on P. patens.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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