Characterization of DNA-strand breakage induced in V79 cells by F 11782, a catalytic inhibitor of topoisomerases
2000
Study of DNA Damage from F 11782 in Cells
publication
Author Information
Author(s): Barret J-M, Hill B T, Olive P L
Primary Institution: Division de Cancérologie, Centre de Recherche Pierre Fabre
Hypothesis
How does F 11782 induce DNA strand breakage in V79 cells compared to other drugs?
Conclusion
F 11782 causes DNA damage that increases over time, but initially induces fewer breaks than other drugs.
Supporting Evidence
- F 11782 produced both DNA single- and double-strand breaks.
- The extent of DNA damage induced by F 11782 increased linearly with time of incubation.
- Proliferating cells were preferentially damaged by F 11782.
Takeaway
This study looked at how a new drug called F 11782 damages DNA in cells. It found that while it starts off causing less damage than other drugs, the damage gets worse the longer the cells are exposed to it.
Methodology
DNA damage was assessed using alkaline and neutral comet assay methodologies.
Digital Object Identifier (DOI)
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