Hipk1 and Its Role in Wnt Signaling During Early Vertebrate Development
Author Information
Author(s): Louie Sarah H., Yang Xiao Yong, Conrad William H., Muster Jeanot, Angers Stephane, Moon Randall T., Cheyette Benjamin N. R.
Primary Institution: University of Washington School of Medicine
Hypothesis
Hipk1 modulates Wnt/β-catenin signaling during early vertebrate development.
Conclusion
Hipk1 contributes to Dsh-dependent signaling activities during early vertebrate development, regulating gene expression and cell movements essential for gastrulation.
Supporting Evidence
- Hipk1 interacts with Dsh and Tcf3, suggesting a role in Wnt signaling.
- Hipk1 knock-down leads to expanded expression domains of Wnt/β-catenin-responsive genes.
- Hipk1 can repress Wnt/β-catenin target gene activation in human embryonic kidney cells.
- Over-expression or knock-down of Hipk1 disrupts convergent extension movements during gastrulation.
Takeaway
Hipk1 is a protein that helps control how cells develop in early embryos by interacting with other proteins involved in signaling pathways.
Methodology
The study used yeast-2-hybrid screening, co-immunoprecipitation assays, and mass spectrometry to analyze protein interactions and functions.
Digital Object Identifier (DOI)
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