Identifying mRNA Targets for the Protein SF2/ASF
Author Information
Author(s): Sanford Jeremy R., Coutinho Pedro, Hackett Jamie A., Wang Xin, Ranahan William, Caceres Javier F.
Primary Institution: MRC Human Genetics Unit, Western General Hospital, Edinburgh, United Kingdom
Hypothesis
SF2/ASF may couple splicing and translation of specific mRNA targets in vivo.
Conclusion
SF2/ASF can co-regulate the nuclear and cytoplasmic processing of specific mRNAs.
Supporting Evidence
- SF2/ASF was found to bind to 326 sites in mRNAs from 180 protein coding genes.
- A purine-rich consensus motif was identified in binding sites located within exon sequences.
- SF2/ASF regulates the splicing and polysome association of transcripts from several genes.
- SF2/ASF remains associated with specific transcripts as they move from the nucleus to the cytoplasm.
Takeaway
The study found that a protein called SF2/ASF helps control how certain messages in our cells are made and used, like a traffic cop for information.
Methodology
The study used Cross-Linking Immunoprecipitation (CLIP) to identify mRNA targets of SF2/ASF in different cellular compartments.
Limitations
The study's findings may not represent all possible RNA targets due to the inefficiency of the sequencing method used.
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website