Functional analysis of the translation factor aIF2/5B in the thermophilic archaeon Sulfolobus solfataricus
2007
Study of aIF2/5B in Sulfolobus solfataricus
publication
Evidence: moderate
Author Information
Author(s): Maone Enzo, Di Stefano Michele, Berardi Alessandra, Benelli Dario, Marzi Stefano, La Teana Anna, Londei Paola
Primary Institution: Università di Roma Sapienza
Hypothesis
What is the role of the archaeal homologue aIF2/5B in translation initiation?
Conclusion
The aIF2/5B protein enhances the binding of initiator tRNA to ribosomes and stimulates translation of both leadered and leaderless mRNAs.
Supporting Evidence
- aIF2/5B is a ribosome-dependent GTPase that stimulates initiator tRNA binding.
- aIF2/5B enhances translation of leadered and leaderless mRNAs.
- Chimeric proteins showed differences in ribosome binding and GTPase activity.
Takeaway
This study found that aIF2/5B helps the ribosome grab the starting piece of protein, making it easier to start making proteins.
Methodology
The function of aIF2/5B was analyzed using in vitro assays, including GTPase activity and ribosome binding experiments.
Digital Object Identifier (DOI)
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