Effects of Antidepressants on BDNF in Human Cells
Author Information
Author(s): Donnici Lorena, Tiraboschi Ettore, Tardito Daniela, Musazzi Laura, Racagni Giorgio, Popoli Maurizio
Primary Institution: University of Milano, Italy
Hypothesis
Do different antidepressants induce changes in the expression of BDNF transcripts in human neuroblastoma cells?
Conclusion
The study found that antidepressants induce biphasic changes in BDNF expression in human cells.
Supporting Evidence
- Short-term treatment with reboxetine or desipramine reduced total BDNF, while long-term treatment significantly increased it.
- Fluoxetine showed no significant effects on BDNF expression.
- BDNF IV and VIa/b transcripts were differentially regulated by the antidepressants.
Takeaway
This study shows that taking certain antidepressants can change the levels of a brain chemical called BDNF in different ways over time.
Methodology
Human neuroblastoma SH-SY5Y cells were treated with antidepressants for varying durations, and BDNF mRNA levels were measured using PCR techniques.
Limitations
BDNF protein levels were not measured, which may not correlate with mRNA levels.
Participant Demographics
Human neuroblastoma SH-SY5Y cells were used, representing a human cell line.
Statistical Information
P-Value
p<0.0001
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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