LPS Increases Hydrogen Peroxide Toxicity in Astrocytoma Cells
Author Information
Author(s): Yue Gang, Shi Guanfang, Azaro Marco A, Yang Qifeng, Hu Guohong, Luo Minjie, Yin Kingsley, Nagele Robert G, Fine Daniel H, Yang Jin-Ming, Li Honghua
Primary Institution: Department of Molecular Genetics, Microbiology and Immunology/The Cancer Institute of New Jersey, University of Medicine and Dentistry of New Jersey
Hypothesis
Does lipopolysaccharide (LPS) cause biological changes in astrocytes that make them vulnerable to reactive oxygen species?
Conclusion
LPS treatment suppresses antioxidant and growth factor gene expression, making astrocytoma cells more sensitive to hydrogen peroxide-induced cell death.
Supporting Evidence
- LPS treatment caused a broad alteration in gene expression profile without obvious cell death.
- Cell death was dramatically increased in LPS pretreated samples after exposure to hydrogen peroxide.
- Down-regulated expression of antioxidant genes was highly correlated with increased cell death.
Takeaway
When brain cells are exposed to a substance from bacteria called LPS, they become weaker and can die more easily when exposed to another harmful substance, hydrogen peroxide.
Methodology
The study used a gene expression profiling system to analyze the effects of LPS on T98G astrocytoma cells and their response to hydrogen peroxide.
Limitations
The study primarily focuses on in vitro conditions, which may not fully replicate in vivo responses.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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