Improved Tumor Response with Actinomycin D and TNF-alpha in Rats
Author Information
Author(s): Seynhaeve A L B, de Wilt J H W, van Tiel S T, Eggermont A M M, ten Hagen T L M
Primary Institution: Department of Surgical Oncology, University Hospital Rotterdam Dijkzigt/Daniel den Hoed Cancer Center, Rotterdam, the Netherlands
Hypothesis
Isolated limb perfusion with actinomycin D and TNF-alpha will improve tumor response in soft-tissue sarcoma-bearing rats.
Conclusion
The combination of actinomycin D and TNF-alpha leads to a tumor response but also causes severe local toxicity.
Supporting Evidence
- Isolated limb perfusion with actinomycin D and TNF-alpha resulted in a 40% tumor response rate.
- Severe local toxicity was observed, including muscle tissue destruction and massive edema.
- Previous studies showed that TNF-alpha combined with melphalan had better response rates.
Takeaway
When rats with tumors were treated with a mix of two drugs, they had some success in shrinking the tumors, but it also caused a lot of damage to the surrounding tissue.
Methodology
Rats were perfused with actinomycin D and TNF-alpha, and tumor responses were measured along with local toxicity assessments.
Potential Biases
Potential bias in the selection of drug concentrations and the animal model used.
Limitations
The study was limited to a rat model and the severe local toxicity may not be applicable to human patients.
Participant Demographics
Male inbred BN rats, weighing 250–300 g.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website