Estrogens Reduce Oxidative Stress and Affect Osteoblasts Without Binding to DNA
Author Information
Author(s): Almeida Maria, Martin-Millan Marta, Ambrogini Elena, Bradsher Robert III, Han Li, Chen Xiao-Dong, Roberson Paula K, Weinstein Robert S, O'Brien Charles A, Jilka Robert L, Manolagas Stavros C
Primary Institution: University of Arkansas for Medical Sciences
Hypothesis
Estrogens attenuate oxidative stress and the differentiation and apoptosis of osteoblasts through non-DNA-binding actions of the estrogen receptor alpha (ERα).
Conclusion
Estrogens can reduce oxidative stress and influence osteoblast behavior without needing to bind to DNA.
Supporting Evidence
- Estrogens reduce the generation of reactive oxygen species in bone.
- Estrogens prevent apoptosis of osteoblasts in both wild-type and ERαNERKI/− mice.
- Estrogens attenuate BMP-2-induced osteoblast differentiation.
- Estrogens activate ERKs and phosphorylate Smad1, leading to decreased osteoblastogenesis.
Takeaway
Estrogens help keep bones healthy by reducing stress in bone cells and preventing them from dying, and they can do this without directly interacting with DNA.
Methodology
The study used a mouse model with an estrogen receptor alpha knock-in mutation and various osteoblast progenitor cell models to investigate the effects of estrogens.
Limitations
The study does not explore the potential effects of estrogen receptor beta (ERβ) or other signaling pathways.
Participant Demographics
The study involved C57BL/6 mice and ERαNERKI/− mice.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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